Fathead minnow fhm cells

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August undefined, 2024

WebThe resulting superna-tant was stored overnight at 4 °C and inoculated onto epithelioma papulosum cyprini (EPC) and fathead minnow (FHM) cell lines cultured in min-imum essential medium (Gibco) supplemented with 10% foetal bovine serum (FBS) (Fryer & Lannan 1994). Cell cultures were incubated at 20 °C; when a cytopathic effect (CPE) … WebJun 7, 2024 · 46 apoptosis in transfected fathead minnow (FHM) cells was investigated. Here, we confirmed that 47 ORF3141 was a secreted protein that targeted host cell mitochondria and induced cell apoptosis in

Fathead Minnow - Lake Superior Streams

WebFathead Minnows. Fathead minnows are a common source of additional food for fish. Fatheads can tolerate lower oxygen levels than most game fish. They have a short life … WebJul 3, 2024 · The cell line designated FHM, originating from tissue of the northern fathead minnow (Pimephales promelas) (Gravell and Malsberger 2010). Until lately, FHM cells … massey\u0027s macclesfield

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WebMar 11, 2024 · In fish cells, there are a handful of carefully controlled experiments with convincing evidence of gene knockdown driven by shRNA-produced by a plasmid. Xie et al. provided convincing data on the fathead minnow (FHM) cell line at 29°C by using co-transfection experiment with a lacZ- and corresponding shRNA-expressing plasmids. … WebThe fathead minnow Pimephales promelas Rafinesque is a member of the fish family Cyprinidae, the largest family of fish with more than 2,000 species worldwide and nearly … WebFathead minnows are found in every drainage in Minnesota. It is the most common species of minnow in the state. They live in many kinds of lakes and streams, but are especially … massey\\u0027s lunch buffet

Digital Atlas of Fathead Minnow Histology: …

Isolation and characterization of plasma-derived …

WebDec 16, 2006 · Two fish cell lines, grouper spleen (GS) (Qin et al., 2006) and fathead minnow (FHM) epithelial cells (Gravell and Malsberger, 1965), were grown in Leibovitz's L15 medium and M199 medium ... WebJul 1, 1993 · The xenobiotics were applied at different concentrations to cultured fathead minnow (FHM) fish cells. After 2 hr contact time the reduction of the total protein content was measured. The results are quantified by the PI 50 value, which is the concentration of test compound required to induce a 50% reduction in total protein content. hydroline service agWebThe liver is a largest of the extramural organs. It is roughly U-shaped, situated ventral to the esophagus and conforming to the peritoneal cavity and surrounding viscera. The color varies from dark brown to cream or even yellow. Functions of the liver include assimilation of nutrients, production of bile, detoxification, hematopoiesis, and ... hydroline pools boca raton

"WebJun 1, 2024 · The pEGFP-N1 plasmids with correct insertions were transfected into fathead minnow (FHM) cells (ATCC, USA). For this, the FHM cells were first seeded in 6-well plates (1 × 10 5 cells/well) at 25 °C in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin, and they were … " - Fathead minnow fhm cells

Fathead minnow fhm cells

WebJun 12, 2014 · The effect of low temperature on VHSV has been shown in several models such as fathead minnow (FHM) cells, rainbow trout, and olive flounder [24][25][26]. WebApr 13, 2024 · 细胞核萎缩、核染色质边缘化，线粒体肿胀、坏死、嵴断裂，细胞质空泡化。取患病鲫组织匀浆滤菌后接种到鲤上皮瘤细胞系 (epithelioma papulosum cyprinid cell line，EPC) 和胖头鲤肌肉细胞系 (fathead minnow cell line, FHM)，盲传 3 代后未见细胞病 …

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WebAug 1, 1987 · CONCLUSION The fathead minnow FHM cell line was suitable for use in the neutral red, cell protein, and cell detachment assays, but not in a colony-formation … WebAug 23, 2016 · The present study utilizes the OECD 210 Early-life Stage (ELS) study to investigate the impacts levonorgestrel (LNG), a synthetic progestin, on fathead minnow (FHM) survival and growth.

WebAbstract. The viral susceptibility range of a poikilothermic cell line derived from the fathead minnow (Pimephales promelas) (FHM) to infection by a number of homoiothermic … WebMoreover, exosomes exhibited immunomodulatory properties associated with wound healing, based on mRNA expression patterns in fathead minnow (FHM) cells. In …

Web（ Oncorhynchus mykiss）性腺细胞系（ RTG － 2 ，是被公认的第一株真骨鱼类的永生性细胞系。随后 Gravell [4 ] Maisberger 1965 和于建立了另一株鱼类细胞系—胖头鲤（ Fathead minnow ）肌肉细胞系（ FHM）。鱼类细胞系的建立表明鱼类体外培养技术的成 … WebJun 9, 2011 · In contrast, SGIV induced typical apoptosis in non-host (fathead minnow, FHM) cells, as evidenced by caspase activation and DNA fragmentation, suggesting that SGIV infection induced nonapoptotic cell death by a cell type dependent fashion. Furthermore, viral replication was essential for SGIV induced nonapoptotic cell death, …

WebSep 21, 2024 · In order to determine the subcellular localization and study the preliminary function of SOD from N. seriolae (NsSOD), gene cloning, secreted protein identification, subcellular localization in fish cells, and apoptosis detection of NsSOD were carried out in this study. Subcellular localization research revealed that NsSOD-GFP fusion proteins ...

WebIn addition, subcellular localization of NsALD-GFP recombinant protein in fathead minnow (FHM) cells showed that the strong green fluorescence co-localized with mitochondria. … massey\\u0027s lawn care serviceWeb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 massey\u0027s lawn care serviceWebJul 3, 2024 · The cell line designated FHM, originating from tissue of the northern fathead minnow (Pimephales promelas) (Gravell and Malsberger 2010). Until lately, FHM cells are able to evaluate the oxicity of methyl mercury (Devlin and Clary 1998 ), the LSER molecular parameters for acute toxicity prediction (Lyakurwa et al. 2014 ), and the influence of ... massey\u0027s meafordWebonto a preformed monolayer of Vero cells. In 2001, fathead minnow (FHM), white sturgeon skin (WSS), epithelioma papillosum caprini (EPC), and channel catfish ovary (CCO) cells were used instead of the Vero cells. Inoculated cells were incubated in a 5% CO2 atmosphere at 37°C. Cells were examined each day for viral cytopathic effect (CPE). massey\\u0027s meafordWebThe samples were inoculated on to cells of the fathead minnow cell line (FHM), incubated at 30 degrees C and observed for 10 days. Cytopathic effect was noted. Medium from cultures were then passaged onto a new cultures for a blind passage (on cultures that had no cytopathic effect) and to confirm infectivity (on cultures that showed cytopathic ... massey\u0027s lunch buffetWeb@article{osti_5427784, title = {Fathead minnow FHM cells for use in in vitro cytotoxicity assays of aquatic pollutants}, author = {Babich, H and Borenfreund, E}, abstractNote = … massey\\u0027s men shoesWebThe Fathead Minnow was collected in 926 bioassessment sampling sessions and 147 fisheries assessment sessions. It was present in 96 rapid bioassessment sessions and … massey\\u0027s mattress sets