In vitro mammalian chromosome aberration test
WebProposal for updating test guideline 473 (2012) OECD 473— In vitro Mammalian Chromosome Aberration Test. Adopted: 21st July 1997. Pfuhler S, Fellows M, Benthem JV et al (2011) In vitro genotoxicity test approaches with better predictivity: summary of an IWGT workshop. Mutat Res 723 (2):101–107. WebJul 29, 2016 · The in vitro chromosome aberration test may employ cultures of established cell lines, cell strains or primary cell cultures. Cell cultures are exposed to the test …
In vitro mammalian chromosome aberration test
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WebChromosome damage is a very important indicator of genetic damage relevant to environmental and clinical studies. Detailed descriptions of the protocols used for detection of chromosomal aberrations induced by genotoxic agents in vitro both in the presence or absence of rat liver-derived metabolizing systems are given in this chapter. WebSeveral in vitro mammalian cell systems are widely used and can be considered sufficiently validated: the in vitro metaphase chromosome aberration assay, the in vitro micronucleus assay
WebThe in vitro mammalian chromosomal aberration test is carried out according to the OECD Guideline 473 1. Proliferating mammalian cell cultures (e.g. Chinese Hamster Ovary cell line CHO or CHO-K1, Human Peripheral Blood Lymphocytes HPBL) are exposed to the test chemical both with and without an exogenous source of metabolic activation (Aroclor ... WebIn vitro mammalian chromosome aberration test (OECD 473) In vitro mammalian cell gene mutation test (OECD 476) Bacterial reverse mutation tests (OECD 471) 15. The classification of individual substances should be based on the total weight of evidence available, using expert judgement. In those instances where a single well-conducted test is ...
WebReport on of the Micronucleus Test in vitro 5 Nominated Use Once validated the micronucleus test (MNT) in vitro is meant to be an alternative or replacement test method for the chromosome aberration test (CAT) in vitro (OECD TG 473; EC Annex V B.10). In the long term, the goal is that the MNT in vitro replaces the MNT in vivo. WebThis test identifies agents that cause structural chromosome aberrations in cultured mammalian somatic cells.
WebJan 1, 2024 · In vitro mammalian chromosome aberration test (Test Guideline [TG] 473). The cells are plated and treated with the metabolic activator (A), along with the test compound (B). This is followed by exposure of cells to colcemid/nocodazole (C), which allows arrest at metaphase. Various chromosomal type aberration (D) and chromatid type …
WebOne mammalian in organism chromosome defocus test is used for the detection of structural chromosome aberrations incited via test compounds in bone marrow measuring in animals, common predatory (rats, mice and Chinese hamsters). pumpkin box pokemon uniteWebTest Type: In vitro mammalian cell gene mutation test Test system: Chinese hamster ovary cells Result: negative Test Type: Chromosome aberration test in vitro Test system: Human lymphocytes Result: negative Method: OECD Test Guideline 486 Species: Rat - … pumpkin businessWebchromosome aberration assays in vitro. While aneugens can induce polyploidy, polyploidy alone does not indicate aneugenic potential and can simply indicate cell cycle … pumpkin axleWebFour end point genotoxicity tests, ie, the bacterial mutation assay, in vitro chromosomal aberration test, in vivo comet assay, and in vivo micronucleus test, were conducted following the test guidelines of the Organization for Economic Cooperation and Development (OECD) with application of Good Laboratory Practice.Results: No … pumpkin bacon pasta jamie oliverWebChromosome Aberration Test Chromosome Aberration Test in vitro The purpose of the in vitro chromosome aberration (CA) test is to identify agents that cause structural chromosome aberrations (clastogenesis) 2)3)4) which are produced by … pumpkin bhajiWebThe in vitro chromosome aberration test may employ cultures of established cell lines, cell strains or primary cell cultures. Cell cultures are exposed to the test substance (liquid or … pumpkin axeWebIn vitro chromosome aberration test: Chinese hamster ovary or Chinese hamster lung fibroblast (V79) cells, hypoxanthine-guanine phosphoribosyl transferase (hgprt) gene locus, accompanied by an appropriate in vitro test for clastogenicity;; In vitro mammalian cell gene mutation test: Mouse lymphoma L5178Y cells, thymidine kinase (tk) gene locus, with … pumpkin bible lesson on salvation